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  • Carbon nanotube wiring for signal amplification of electrochemical magneto immunosensors. Application to myeloperoxidase detection
    for signal amplification of electrochemical magneto immunosensors Application to myeloperoxidase detection Analytical and Bioanalytical Chemistry 2014 Sep 406 22 5487 93 Herrasti Zorione Martínez Fernando Baldrich Eva In this work chronoamperometric myelo peroxidase MPO detection was accomplished using immunofunctionalized magnetic microparticles MPs disposable carbon screen printed electrodes C SPEs and a ready to use commercially available tetramethylbenzidine TMB based enzymatic substrate In order to reach the limit of detection LOD needed to study real blood serum samples assay performance was additionally improved by exploiting CNT wiring which amplified the signal and decreased the LOD The optimized assay can be performed in 30 min and yields LODs of 6 and 55 ng mL 1 in PBS and undiluted human serum respectively making it useful for the identification of patients at risk of cardiovascular disease These results demonstrate that electrode nanostructuring can be accomplished post assay which favors the development of enhanced magneto immunosensors based on the exploitation of cheap and simple SPE devices IK4 Ikerlan Organisation and team Results Infrastructures and equipment Professional development IK4 Research Alliance Mondragon Corporation R D for companies Product and service development Energy efficiency and energy storage Operational efficiency and advanced manufacturing IK4 IKERLAN Inspires Our

    Original URL path: http://www.ikerlan.es/en/news/papers-theses/carbon-nanotube-wiring-for-signal-amplification-of-electrochemical-magneto-immunosensors-application-to-myeloperoxidase-detection (2016-02-15)
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  • Electrochemical biosensing of non-electroactive targets using ferrocene-labeled magnetic particles and CNT wiring
    enhances ten fold the signals registered compared to direct detection of the MPs alone As a proof of concept detection of detergents and antibodies is accomplished In these preliminary experiments random binding of 0 01 Tween 20 onto MP Fc was detectable both voltametrically and impedimetrically after a 2 min incubation Furthermore affinity capture of 4 μg mL 1 of biotinylated antibody by streptavidin MP Fc could be measured in

    Original URL path: http://www.ikerlan.es/en/news/papers-theses/electrochemical-biosensing-of-nonelectroactive-targets-using-ferrocenelabeled-magnetic-particles-and-cnt-wiring (2016-02-15)
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  • SU-8 based microprobes for simultaneous depth neural recording and drug delivery in the brain
    la Prida Anton Guimerá Javier Berganzo Gemma Gabriel Rosa Villa and Luis J Fernández While novel influential concepts in neuroscience bring the focus to local activities generated within a few tens of cubic micrometers in the brain we are still devoid of appropriate tools to record and manipulate pharmacologically neuronal activity at this fine scale Here we designed fabricated and encapsulated microprobes for simultaneous depth recording and drug delivery using exclusively the polymer SU 8 as structural material A tetrode and linear like lectrode patterning was combined for the first time with single and double fluidic microchannels for independent drug delivery The device was tested experimentally using the in vivo anesthetized rat preparation Both probe types successfully recorded detailed spatiotemporal features of local field potentials and single cell activity at a resolution never attained before with integrated fluidic probes Drug delivery was achieved with high spatial and temporal precision in a range from tens of nanoliters to a few microliters as confirmed histologically These technological advancements will foster a wide range of neural applications aimed at simultaneous monitoring of brain activity and delivery at a very precise micrometer scale IK4 Ikerlan Organisation and team Results Infrastructures and equipment Professional development

    Original URL path: http://www.ikerlan.es/en/news/papers-theses/su8-based-microprobes-for-simultaneous-depth-neural-recording-and-drug-delivery-in-the-brain (2016-02-15)
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  • Pre-storage of gelified reagents in a lab-on-a-foil system for rapid nucleic acid analysis
    1509 1514 Yi Sun Jonas Høgberg Thanner Christine Laouenan Florian Lisandro G Monsalve Sonia Rodriguez Cuong Cao Anders Wolff Jesus M Ruano Lopez and Dang Duong Bang Reagent pre storage in a microfluidic chip can enhance operator convenience simplify the system design reduce the cost of storage and shipment and avoid the risk of cross contamination Although dry reagents have long been used in lateral flow immunoassays they have rarely been used for nucleic acid based point of care POC assays due to the lack of reliable techniques to dehydrate and store fragile molecules involved in the reaction In this study we describe a simple and efficient method for prolonged on chip storage of PCR reagents The method is based on gelification of all reagents required for PCR as a ready touse product The approach was successfully implemented in a lab on a foil system and the gelification process was automated for mass production Integration of reagents on chip by gelification greatly facilitated the development of easy to use lab on a chip LOC devices for fast and cost effective POC analysis IK4 Ikerlan Organisation and team Results Infrastructures and equipment Professional development IK4 Research Alliance Mondragon Corporation R D

    Original URL path: http://www.ikerlan.es/en/news/papers-theses/prestorage-of-gelified-reagents-in-a-labonafoil-system-for-rapid-nucleic-acid-analysis (2016-02-15)
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  • Real-time isothermal RNA amplification of toxic marine microalgae using preserved reagents on an integrated microfluidic platform
    602 DOI 10 1039 C2AN36464F Maria Nefeli Tsaloglou Florian Laouenan Christos Moritz Loukas Lisandro Gabriel Monsalve Christine Thanner Hywel Morgan Jesus M Ruano López and Matthew C Mowlem Quantitation of specific RNA sequences is a useful technique in marine biology that can elucidate cell abundance speciation and viability especially for early detection of harmful algal blooms We are thus developing an integrated microfluidic system for cell concentration and lysis RNA extraction purification and quantitative RNA detection for environmental applications The portable system is based on a microfluidic cartridge or lab card using a low cost injection moulded device with a laminated lid Here we present real time isothermal RNA amplification using reagent master mixes preserved on chip in a gel at 4 C for up to eight months We demonstrate quantitation by reference to an internal control in a competitive assay with 500 cell equivalents of the toxic microalga Karenia brevis Annealing of primers amplification at 41 C and real time fluorescence detection of the internal control and target using sequence specific molecular beacons were all performed on chip IK4 Ikerlan Organisation and team Results Infrastructures and equipment Professional development IK4 Research Alliance Mondragon Corporation R D for companies Product

    Original URL path: http://www.ikerlan.es/en/news/papers-theses/realtime-isothermal-rna-amplification-of-toxic-marine-microalgae-using-preserved-reagents-on-an-integrated-microfluidic-platform (2016-02-15)
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  • Surface treatment patterning of organic photovoltaic films for low-cost modules
    2013 430 435 Ikerne Etxebarria J G Tait R Gehlhaar R Pacios D Cheyns This work describes a patterning technique for the photoactive layer of organic photovoltaic modules We demonstrate the fabrication of efficient poly 3 hexyl thiophene 2 5 diyl 6 6 phenyl C61 butyric acid methyl ester P3HT PCBM based organic photovoltaic modules through a specific surface treatment based on the deposition of a fluorinated self assembled monolayer SAM on top of the bottom electric contact Direct self patterning of the photoactive layer is achieved by the high contact angle between the SAM and the polymer solution while a smooth topography is created by combining two solvents with different surface tensions and boiling points in the polymer PCBM solution The resolution of the patterning is approximately 400 lm for modules based on a conventional cell architecture and 120 lm for an inverted architecture As a result we show 25 cm2 P3HT PCBM based organic photovoltaic modules with 10 series connected cells fabricated via roll to roll compatible deposition and patterning techniques IK4 Ikerlan Organisation and team Results Infrastructures and equipment Professional development IK4 Research Alliance Mondragon Corporation R D for companies Product and service development Energy efficiency and

    Original URL path: http://www.ikerlan.es/en/news/papers-theses/surface-treatment-patterning-of-organic-photovoltaic-films-for-lowcost-modules (2016-02-15)
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  • Nanomorphology influence on the light conversion mechanisms in highly efficient diketopyrrolopyrrole based organic solar cells
    14 2013 326 334 Jon Ajuria Sudam Chavhan Ramón Tena Zaera Jihua Chen Adam J Rondinone Prashant Sonar Ananth Dodabalapur Roberto Pacios In this work diketopyrrolopyrrole based polymer bulk heterojunction solar cells with inverted and regular architecture have been nvestigated The influence of the polymer fullerene ratio on the photoactive film nanomorphology has been studied in detail Transmission Electron Microscopy and Atomic Force Microscopy reveal that the resulting film morphology strongly depends on the fullerene ratio This fact determines the photocurrent generation and governs the transport of free charge carriers Slight variations on the PCBM ratio respect to the polymer show great differences on the electrical behavior of the solar cell Once the polymer fullerene ratio is accurately adjusted power conversion efficiencies of 4 7 and 4 9 are obtained for inverted and regular architectures respectively Furthermore by correlating the optical and morphological characterization of the polymer fullerene films and the electrical behavior of solar cells an ad hoc interpretation is proposed to explain the photovoltaic performance as a function of this olymer blend composition IK4 Ikerlan Organisation and team Results Infrastructures and equipment Professional development IK4 Research Alliance Mondragon Corporation R D for companies Product and service development Energy

    Original URL path: http://www.ikerlan.es/en/news/papers-theses/nanomorphology-influence-on-the-light-conversion-mechanisms-in-highly-efficient-diketopyrrolopyrrole-based-organic-solar-cells (2016-02-15)
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  • Development and integration of an electrochemical system ina LOC device for DNA detection
    of an electrochemical system ina LOC device for DNA detection Procedia Engineering 47 2012 25 28 Z Herrasti I Etxabe J M Mitxelena M P Martínez F Martínez In this work we have developed an electrochemical detection system EDS with the aim to simplify and reduce the cost of a Lab on a Chip LOC device 1 in the case of qualitative DNA detection measurements For that in addition to the optical detection system of the LOC device an EDS is added The LOC system consists of a potable platform reader actuator and a disposable labcard capable of performing nucleic acid concentration and amplification The sample is injected in the labcard and after the elution of the DNA it is transferred to the PCR chamber where the DNA is amplified and transferred to the detection chamber In this chamber an electrochemical sensor is integrated In addition a potentiostat galvanostat which is integrated in the LOC system is developed for the electrochemical measurements IK4 Ikerlan Organisation and team Results Infrastructures and equipment Professional development IK4 Research Alliance Mondragon Corporation R D for companies Product and service development Energy efficiency and energy storage Operational efficiency and advanced manufacturing IK4 IKERLAN Inspires Our

    Original URL path: http://www.ikerlan.es/en/news/papers-theses/development-and-integration-of-an-electrochemical-system-ina-loc-device-for-dna-detection (2016-02-15)
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