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  • Directory - iib.uam.es
    Directory Directory IIBM Directory Service Aragón Reyes Juan José Category Catedrático Laboratory Laboratory of Juan José Aragón Reyes B 45 Department Department of Metabolism and Cell Signaling Phone Number 34 91 497 5333 Fax 34 91 497 5353 34 91 585 4401 E Mail jjaragon Publications View publications New Search Back Navigation Presentation Welcome Location History Biography of Alberto Sols Organization Organization chart Management Administration Institute Board Faculty Board Committees

    Original URL path: https://www.iib.uam.es/portal/en/personal?p_p_id=APPERSportlet_WAR_APIIBportlet_INSTANCE_vAb1&p_p_lifecycle=0&p_p_col_id=column-3&p_p_col_count=1&_APPERSportlet_WAR_APIIBportlet_INSTANCE_vAb1_idPersona=9&_APPERSportlet_WAR_APIIBportlet_INSTANCE_vAb1_action=detail_person (2015-08-08)
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  • Research Groups - iib.uam.es
    Department Department of Endocrine and Nervous System Pathophysiology Department of Metabolism and Cell Signaling Department of Experimental Models of Human Disease Scientific Reports Scientific Reports in pdf format Publications Laboratory of Juan José Aragón Reyes Staff Head of Group Aragón Reyes Juan José Contratado Doctor Hermida Díaz Carmen Técnico Medio Sánchez López Valentina Introduction Staff Research topics Publications Additional Information Navigation Presentation Welcome Location History Biography of Alberto Sols Organization

    Original URL path: https://www.iib.uam.es/portal/en/investigacion/grupos?p_p_id=APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq&p_p_lifecycle=0&p_p_state=normal&p_p_mode=view&p_p_col_id=column-3&p_p_col_count=1&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_action=detail&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_id=55&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_idJefe=9&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_menu=miembros (2015-08-08)
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  • Research Groups - iib.uam.es
    form the active site since expression of this half from either the human muscle or the Dictyostelium discoideum enzyme restores growth in glucose from a strain of yeast deficient in PFK Nevertheless the recombinant enzyme is not stable in vitro The C terminal half is by contrast not catalytic but is actually needed for stability of the tetramer as is the connecting peptide that normally joins the two domains Co expression of homologous but not heterologous N and C termini yielded a fully active enzyme in vitro with kinetic and physical properties similar to those of the wild type tetrameric enzymes This indicates that binding of the separately translated domains is sufficiently accurate and tight as to preserve the metabolite binding sites and allosteric interactions Biochem J 445 213 8 2012 Control of mammalian PFK gene expression in response to glucose Lázaro A Hermida C Aragón JJ Martínez Costa OH It is known that glucose independently of insulin induces hepatic expression of lipogenic and glycolytic enzymes such as pyruvate kinase L as it occurs in carbohydrate fed animals and in isolated hepatocytes incubated with high concentrations of glucose The effect on pyruvate kinase L and glyceraldehyde 3 P dehydrogenase has also been described in pancreatic beta cells In these cells we have found that high extracellular glucose triggers a marked increase in PFK enzyme activity as well as in protein and mRNA levels of the three PFK subunits C M and L Since glycolysis plays a key role in the interaction between hyperglycaemia and insulin secretion and PFK activity is essential for the control of the glycolytic pathway we seek to investigate the underlying molecular mechanisms involved in glucose response of PFK genes in pancreatic beta cells Our in vivo and in vitro analyses on PFK C gene promoter allowed us to conclude that the transcription factor ChREBP a key regulator for the induction of pyruvate kinase L and other enzymes in response to glucose by its binding to ChoRE elements also regulates the expression of PFK C gene constituting a new gene target for this factor Overexpression of ChREBP or the dominant negative R673A R674G of this factor in beta cells does not affect however the activity of PFK C promoter containing either one or more of its ChoREs nor that of the PK L promoter considered a model of glucose mediated transcriptional induction Immunoblot analysis showed that although total ChREBP levels were reduced with increasing extracellular glucose the amounts of the transcriptional factor augmented in the nucleus while decreased in the cytoplasm This indicates that ChREBP effects depend much more on its intranuclear activation than on its intracellular levels Despite being the minor isoform in beta cells PFK L gene expression showed the most prominent increase in response to glucose Thus we undertook the functional characterization of PFK L glucose dependent induction which in turn could be extrapolated to other tissues e g liver where expression of PFK L is predominant In the region of maximum promoter activity

    Original URL path: https://www.iib.uam.es/portal/en/investigacion/grupos?p_p_id=APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq&p_p_lifecycle=0&p_p_state=normal&p_p_mode=view&p_p_col_id=column-3&p_p_col_count=1&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_action=detail&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_id=55&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_idJefe=9&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_menu=lineas (2015-08-08)
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  • Research Groups - iib.uam.es
    Department of Metabolism and Cell Signaling Department of Experimental Models of Human Disease Scientific Reports Scientific Reports in pdf format Publications Laboratory of Juan José Aragón Reyes Publications Scientific Report 2011 2012 Scientific Report 2008 2010 Scientific Report 2006 2007 Scientific Report 2004 2005 Scientific Report 2002 2003 Scientific Report 2000 2001 All publications of the principal investigator View publications Back Introduction Staff Research topics Publications Additional Information Navigation Presentation

    Original URL path: https://www.iib.uam.es/portal/en/investigacion/grupos?p_p_id=APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq&p_p_lifecycle=0&p_p_state=normal&p_p_mode=view&p_p_col_id=column-3&p_p_col_count=1&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_action=detail&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_id=55&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_idJefe=9&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_menu=public (2015-08-08)
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  • Research Groups - iib.uam.es
    and Cell Signaling Department of Experimental Models of Human Disease Scientific Reports Scientific Reports in pdf format Publications Laboratory of Juan José Aragón Reyes Additional Information Department the Laboratory belongs to Department of Metabolism and Cell Signaling Scientific Reports available Scientific Reports 2011 2012 Scientific Reports 2008 2010 Scientific Reports 2006 2007 Scientific Reports 2004 2005 Scientific Reports 2002 2003 Scientific Reports 2000 2001 Introduction Staff Research topics Publications Additional

    Original URL path: https://www.iib.uam.es/portal/en/investigacion/grupos?p_p_id=APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq&p_p_lifecycle=0&p_p_state=normal&p_p_mode=view&p_p_col_id=column-3&p_p_col_count=1&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_action=detail&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_id=55&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_idJefe=9&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_menu=info (2015-08-08)
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  • Directory - iib.uam.es
    es Directory Directory IIBM Directory Service Gancedo Rodríguez Carlos Category Dr Ad Honorem Laboratory Laboratory of Carlos Gancedo Rodríguez 1 1 2 Department Department of Metabolism and Cell Signaling Phone Number 34 91 585 4431 Fax 34 91 585 4401 E Mail cgancedo Publications View publications New Search Back Navigation Presentation Welcome Location History Biography of Alberto Sols Organization Organization chart Management Administration Institute Board Faculty Board Committees Commissions Research

    Original URL path: https://www.iib.uam.es/portal/en/personal?p_p_id=APPERSportlet_WAR_APIIBportlet_INSTANCE_vAb1&p_p_lifecycle=0&p_p_col_id=column-3&p_p_col_count=1&_APPERSportlet_WAR_APIIBportlet_INSTANCE_vAb1_idPersona=77&_APPERSportlet_WAR_APIIBportlet_INSTANCE_vAb1_action=detail_person (2015-08-08)
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  • Research Groups - iib.uam.es
    Departments Cancer Biology Department Department of Endocrine and Nervous System Pathophysiology Department of Metabolism and Cell Signaling Department of Experimental Models of Human Disease Scientific Reports Scientific Reports in pdf format Publications Laboratory of Carlos Gancedo Rodríguez Staff Head of Group Gancedo Rodríguez Carlos Técnico Superior Flores Mauriz Carmen Lisset Introduction Staff Research topics Publications Additional Information Navigation Presentation Welcome Location History Biography of Alberto Sols Organization Organization chart Management

    Original URL path: https://www.iib.uam.es/portal/en/investigacion/grupos?p_p_id=APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq&p_p_lifecycle=0&p_p_state=normal&p_p_mode=view&p_p_col_id=column-3&p_p_col_count=1&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_action=detail&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_id=17&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_idJefe=77&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_menu=miembros (2015-08-08)
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  • Research Groups - iib.uam.es
    may be due to the peculiar chromosomal position of YlTPS1 A mapping of the neighbouring genes has shown that in Y lipolytica the promoter of the essential gene TFC1 overlaps with the coding sequence of YlTPS1 a position that is not found in other species examined We think that this situation has influenced the evolution of controls on YlTPS1 expression We have shown that the growth in glucose of a Yltps1 mutant in contrast with the situation in S cerevisiae is due to differences between these yeasts in the enzymatic equipment for glucose phosphorylation We have cloned the genes encoding the glucose phosphorylating enzymes in Y lipolytica and shown that glucokinase and not hexokinase is the majoritary enzyme Glucokinase is not inhibited by trehalose 6 P therefore a mutation in YlTPS1 has no effect on its phosphorylation capacity We have also initiated studies on other kinases for unusual sugars with potential importance in the glycosylation of proteins Catabolite repression in Y lipolytica appears also to follow different patterns from those found in S cerevisiae Previous studies of our group have shown that not all genes encoding gluconeogenic enzymes are repressed by glucose Among those responsive to that repression is YlPCK1 the gene that encodes phosphonelpyruvate carboxykinase Since there is no information on regulatory elements participating in catabolite repression in Y lipolytica we are analyzing the promoter of YlPCK1 to locate regions implicated in this regulation with the ultimate objective of identifying proteins participating in the process Role of the cAMP dependent protein kinases PKAs in the control of transcription by glucose in the yeast Saccharomyces cerevisiae A global analysis of transcription has been performed in yeast strains lacking PKA We found that PKA activity was not required for the induction of genes related with glucose metabolism nor for the repression

    Original URL path: https://www.iib.uam.es/portal/en/investigacion/grupos?p_p_id=APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq&p_p_lifecycle=0&p_p_state=normal&p_p_mode=view&p_p_col_id=column-3&p_p_col_count=1&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_action=detail&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_id=17&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_idJefe=77&_APGIportlet_WAR_APIIBportlet_INSTANCE_2Veq_menu=lineas (2015-08-08)
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